Kisspeptin

Hormones & fertility Investigational (in clinical trials)

Neuropeptide; GPR54 (KISS1R) agonist · also known as Kisspeptin-10, Kisspeptin-54, Metastin

For reference only — not medical advice. Figures are commonly reported values compiled from published sources and may be wrong or out of date. Many compounds listed are not approved for human use. Talk to a licensed clinician before using any compound.

Kisspeptins act on neurons that release GnRH, so they influence LH and FSH secretion indirectly. Academic groups have given kisspeptin-54 and kisspeptin-10 to small groups of volunteers and patients to probe reproductive signaling and as a possible fertility tool. Doses in those studies were usually weight-based and delivered by injection or infusion.

Commonly reported dosing (10 mg vial)

Reconstituted with 3 mL → 3.333 mg/mL.

Phase	Dose	Volume	U-100 units
Starting	100 mcg	0.03 mL	3
Standard	200 mcg	0.06 mL	6

Frequency: Commonly reported once daily · Half-life: Short (minutes; varies by fragment, shorter for kisspeptin-10 than kisspeptin-54)

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Commonly reported dose escalation

Weeks	Dose	Volume	U-100 units
Weeks 1-2	100 mcg	0.03 mL	3
Week 3+	200 mcg	0.06 mL	6

Higher steps may need a larger vial or more than one draw. Escalation schedules vary; dosing decisions belong to a licensed clinician.

Commonly reported reconstitution steps

Vials are usually brought to room temperature (about 15-20 minutes) first.
3 mL of bacteriostatic water is added slowly down the inside wall of the 10 mg vial.
The vial is swirled gently until clear, not shaken.
This gives 3.33 mg/mL. Mixed vials are typically labeled with the date and refrigerated.

Storage

Before mixing: Lyophilized research vials are commonly stored frozen (around -20 °C), dry and protected from light.

After mixing: Reconstituted solution is commonly kept refrigerated (2-8 °C), protected from light, and not frozen; product-specific use-by guidance varies.

How it works

Kisspeptin binds the GPR54 receptor on hypothalamic GnRH neurons, prompting GnRH release and, downstream, LH and FSH secretion. Its effect depends on intact GnRH signaling.

Researched for

Small human studies of kisspeptin-54 in women with hypothalamic amenorrhea, where twice-daily dosing for two weeks led to desensitization while twice-weekly dosing for eight weeks kept raising LH and FSH.
Given to healthy volunteers in small studies to probe reproductive endocrine function.
Registered clinical trials are ongoing; none has led to an approved product.

Notes

Published human work uses weight-based doses of specific fragments (for example kisspeptin-54), so the flat microgram figures in reference charts are not directly equivalent.
Evidence quality is limited to small academic studies.
No approved kisspeptin product exists in the US.

Sources

Last reviewed 2026-10-05.

Gonadorelin

Synthetic GnRH decapeptide

Gonadorelin is the synthetic form of hypothalamic GnRH, the signal that prompts the pituitary to release LH and FSH.

2 mg vial · Commonly reported 2-3 times weekly on non-consecutive days

hCG (Human Chorionic Gonadotropin)

Gonadotropin (LH-receptor agonist glycoprotein hormone)

hCG is a placental glycoprotein hormone that acts on the same receptor as luteinizing hormone, and it is used in fertility and male hypogonadism medicine.

5000 IU vial · Commonly reported three times weekly (for example Mon/Wed/Fri)