TB-500

Healing & recovery Research compound (not approved for human use)

Thymosin beta-4 fragment · also known as TB500, Thymosin beta-4 fragment

For reference only — not medical advice. Figures are commonly reported values compiled from published sources and may be wrong or out of date. Many compounds listed are not approved for human use. Talk to a licensed clinician before using any compound.

TB-500 is a synthetic version of a region of the naturally occurring protein thymosin beta-4. It is studied for its role in cell migration and tissue repair, and is often combined with BPC-157 in research.

Commonly reported dosing (5 mg vial)

Reconstituted with 3 mL → 1.667 mg/mL.

Also sold in 10 mg vials.

Phase	Dose	Volume	U-100 units
Starting Weeks 1-2	500 mcg	0.3 mL	30
Step Weeks 3-4	600 mcg	0.36 mL	36
Standard Weeks 5-8	750 mcg	0.45 mL	45
Higher Weeks 9-12	1 mg	0.6 mL	60

Frequency: Once daily, subcutaneous (a weekly loading pattern is also reported) · Half-life: Not well established in humans

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Commonly reported reconstitution steps

Vials are usually brought to room temperature (about 15-20 minutes) first.
3 mL of bacteriostatic water is added slowly down the inside wall of the 5 mg vial.
The vial is swirled gently until clear, not shaken.
This gives 1.67 mg/mL. Mixed vials are typically labeled with the date and refrigerated.

Storage

Before mixing: Sealed vials are commonly stored refrigerated (2-8 °C) and protected from light, or frozen for long-term storage.

After mixing: Reconstituted solution is commonly kept refrigerated (2-8 °C), protected from light, and not frozen.

Commonly used within 28 days of mixing.

How it works

Thymosin beta-4 regulates actin, a key cytoskeletal protein involved in cell movement. This activity is associated in research with wound healing, new blood vessel formation, and reduced inflammation.

Researched for

Studied for connective-tissue and wound repair in animal models.
Investigated for promoting cell migration during repair.

Notes

Two patterns are commonly reported: a daily staged schedule (500, 600, 750 then 1000 mcg, tabulated here, roughly 5 mg per week on average) and a weekly loading schedule using larger, less frequent amounts of about 2-2.5 mg.
No human dose-ranging trial exists for TB-500 itself; the figures are community-reported.
Reconstituted solution is commonly refrigerated and used within a few weeks.
Often reported alongside BPC-157.

Sources

Last reviewed 2026-10-06.

BPC-157

Synthetic pentadecapeptide

BPC-157 is a synthetic peptide derived from a sequence found in gastric juice.

5 mg vial · Once daily, subcutaneous (some reports split it into two injections)

BPC-157 + TB-500 20 mg Blend

Tissue-repair research blend (BPC-157 + TB-500)

This blend combines BPC-157 and TB-500 in a single vial (20 mg by default, with a 10 mg size also listed), a common pairing in recovery-oriented research because the two peptides are studied for complementary roles in tissue-repair research.

20 mg vial · Once daily, subcutaneous