Humanin

Longevity Research compound (not approved for human use)

Mitochondrial-derived peptide · also known as HN, Humanin G (HNG)

For reference only — not medical advice. Figures are commonly reported values compiled from published sources and may be wrong or out of date. Many compounds listed are not approved for human use. Talk to a licensed clinician before using any compound.

Humanin was found as a factor that protected neurons in Alzheimer's disease models and was later shown to be encoded in mitochondrial DNA. Circulating levels tend to fall with age, which fueled interest in it as a longevity-related signal. Stable analogs such as HNG are the forms most used in experiments.

Commonly reported dosing (10 mg vial)

Reconstituted with 3 mL → 3.333 mg/mL.

Phase	Dose	Volume	U-100 units
Starting	1 mg	0.3 mL	30
Standard	2 mg	0.6 mL	60
Higher	3 mg	0.9 mL	90

Frequency: Twice weekly (community-reported), subcutaneous · Half-life: Not well characterized in humans

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Commonly reported reconstitution steps

Vials are usually brought to room temperature (about 15-20 minutes) first.
3 mL of bacteriostatic water is added slowly down the inside wall of the 10 mg vial.
The vial is swirled gently until clear, not shaken.
This gives 3.33 mg/mL. Mixed vials are typically labeled with the date and refrigerated.

Storage

Before mixing: Sealed vials are commonly stored refrigerated (2-8 °C) and protected from light.

After mixing: Reconstituted solution is commonly kept refrigerated (2-8 °C) and protected from light.

Commonly used within 28 days of mixing.

How it works

Reported actions include binding to cell-surface receptor complexes and intracellular partners, reducing apoptosis signaling, and influencing insulin sensitivity and stress responses.

Researched for

Studied in cell and animal models for neuroprotection, cardiac protection, and metabolic effects.
Reviewed as a mitochondrial signaling peptide whose levels decline with age.

Notes

No controlled human study establishes a dose, route, frequency, or safety profile; the regimen shown is community-reported.
Much of the research uses analogs rather than the native peptide.